KAPA RNA HyperPrep Kit with RiboErase (HMR)

RNA sequencing Mouse - ESCs (Embryonic Stem Cells)

Experiment
RNA sequencing Mouse - ESCs (Embryonic Stem Cells)
Product
KAPA RNA HyperPrep Kit with RiboErase (HMR) from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Protocol tips
Input RNA: 1 ng-100 ng.

Publication protocol

For paRNA-seq, we purified poly(A) RNA from 1 µg of ES total RNA using a NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). For rdRNA-seq, we depleted rRNA from 1 µg of ES total RNA using a GeneRead rRNA Depletion Kit (Qiagen). We prepared conventional RNA-seq library DNA with the resulting RNA using a commercial kit (NEBNext Ultra Directional RNA Library Prep Kit for Illumina; NEB) in accordance with the manufacturer’s protocol, with slight modifications during RT and PCR steps. We used SuperScript III (Thermo Fisher) for RT. We used KAPA HiFi DNA polymerase (Kapa Biosystems) in the PCR step. When we performed rdRNA-seq using 10 or 1 ng of ES total RNA, we utilized a KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems) for the preparation of sequencing library DNA according to the manual instructions.

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Discussion

Discussion

5 years ago

Author: Panagiotis Karagiannis Greece

Using RNA from formalin fixed paraffin embedded tissue sections for sequencing

Hello there! I have some slides with mouse lung tumors and I would like to do rna sequencing. Will I be able to extract enough/good quality rna from RNA from formalin fixed paraffin embedded tissue sections for sequencing to be successful?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Mouse - ESCs (Embryonic Stem Cells) using KAPA RNA HyperPrep Kit with RiboErase (HMR) from Roche Lifesciences.

Paper title
Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for KAPA RNA HyperPrep Kit with RiboErase (HMR) below.

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